Signals VitroVivo: Create a new curve fit from the Manage Fittings of the Calculations Explorer Controls panel

Prior to this task:

✔ Calculations Explorer template must be created.

✔ Calculations Explorer Controls panel must be opened.

1️⃣ Calculations Explorer Controls panel ➡ select Manage Fittings
2️⃣ Manage Fittings tab ➡ select  [Create New Fit]
3️⃣ Global Settings tab ➡ select Fitting Type
4️⃣ Global Settings tab ➡ select Advanced
5️⃣ Advanced tab ➡ select Algorithm Y Weighting Filter by column
6️⃣ Advanced tab ➡ select Columns
7️⃣ Columns tab ➡ Additional points ➡ select/type [EC/IC] concentration [Add Parameter]
8️⃣ Columns tab ➡ select/type [Add coefficient columns] [Add parameter columns] [Add CI columns] [Set EC/IC Abs scale to 0-100] [Add thumbnails column] Thumbnail size Thumbnail row height
9️⃣ Columns tab ➡ Add columns to Fit result ➡ select column(s)
🔟 Columns tab ➡ select  [Ok]

Supporting Knowledge

Optionally, from the Global Settings tab, the user can also select the  [Apply fit settings] button to apply standard curve fit adjustments to quickly perform QA/QC; select the  [Save fit settings] button to save fit settings under a desired name; and select the  [Import fit settings] button to establish the fitting parameters based on an imported local .json file.

Note: On the Advanced tab, the Levenberg-Marquardt Robust algorithm applies an IRLS implementation of the LM to carry out the fitting while down-weighting points that can be possible outliers.

Optionally, the user can also add absolute half-maximal inhibitory concentration or absolute half-maximal effective concentration. From the Columns tab, under Additional points, select AbsEC/AbsIC, and type the desired concentration in the box below; select the [Add Parameter] button to add the new point. For the absolute EC/IC, the user can select a scale. So, from the Columns tab, select the [Set EC/IC Abs scale to 0–100] toggle.

Optionally, the user can add thumbnails to the resulting curve fit. So, from the Columns tab, select the [Add thumbnails column] toggle.

Optionally, the user can observe the new parameters table created with the information of the curve fit visualization by selecting the  [Show/Hide visualization] icon to show the table.

Optionally, from the Calculations Explorer Controls panel, from the Manage Fittings tab, under Select Fit, select the dropdown to navigate between the fits created.