Spectrus: Using J-resolved 2D NMR spectra to separate chemical shift and coupling information

Description
J-resolved 2D NMR spectra separate chemical shift (one axis) from scalar coupling (other axis), simplifying analysis of crowded 1H spectra. Spectrus can display and process J-resolved experiments to support coupling analysis.

Solution

1. Acquire a 2D J-resolved spectrum for the sample (according to instrument documentation).
2. Import the J-resolved dataset into Spectrus and process:
   • Phase and baseline correction in both dimensions.
3. View the spectrum in:
   • Standard 2D mode, and
   • Optional “tilted” or processed forms (if available) where multiplet patterns are more clearly separated.
4. Use cursor tools to:
   • Read chemical shifts along the F2 (1H) axis.
   • Read J values along the F1 (J) axis.
5. Apply this information to:
   • Clarify overlapping multiplets.
   • Assign coupling constants more reliably in complex regions.
6. Combine J-resolved data with 1H, COSY, and HSQC information for complete assignment.