Katalyst: Sequence file missing for Katalyst experiment – impact and recovery options

Description

• Users expect a sequence file associated with a Katalyst experiment for their instrument (e.g., LC/MS).
• The sequence file cannot be found:
  • It was never generated, or
  • It was deleted or moved.
• Subsequent analytical data may have been acquired without using the KAT ID–aware sequence.

Solution

• Assess the impact:
  • If no analytical data has been collected yet:
    • Generate a new sequence file from Katalyst for that experiment and load it into the instrument software before acquiring data.
  • If data has already been collected without the Katalyst sequence:
    • Data files may not contain expected KAT IDs or naming patterns, so automatic attachment may fail.
• Recovery when data exists without the original sequence:
  1. Generate a new sequence file for the experiment in Katalyst.
  2. Compare the new sequence with the actual acquired data:
     • Align samples/wells manually (e.g., by acquisition order) to know which raw file belongs to which well.
  3. Rename the raw data files to match the filenames expected in the new sequence (including KAT IDs).
  4. Move renamed files into the scan folder so Katalyst can attach and process them.
• For future experiments:
  • Always generate and use sequence files from Katalyst before data acquisition to avoid manual remapping steps.