Define Normalization parameters for an In Vitro Analysis template from the Signals Configuration

For Administrators only.

Prior to this task:

✔ In Vitro Analysis template must be created.

✔ Data Assembly must be defined.

*Required.

1️⃣ Signals Configuration ➡ select [System Objects]
2️⃣ System Objects ➡ select In Vitro Analysis
3️⃣ In Vitro Analysis Settings pane ➡ select Templates
4️⃣ In Vitro Analysis Templates section ➡ select in vitro analysis template
5️⃣ In Vitro Analysis Contents ➡ Analytics ➡ select [Normalization]
6️⃣ Normalization section ➡ select [Configure]
7️⃣ Configure Normalization window ➡ select Feature Column ➡ select Read 1* data calculation Normalization ➡ select Intra Plate Method Inter Plate Method Controls
8️⃣ Configure Normalization window ➡ select [Save]

Supporting Knowledge

† Normalization methods available:

• Intra Plate Method: none, Median polish.
• Inter Plate Method: none, Median, Normalized percent activation (NPA) (ratio), Normalized percent inhibition (NPI) (ratio), Normalized percent activation (NPA), Normalized percent inhibition (NPI), Reversed NPI (ratio), Robust Normalized Percent Activation (NPA), Robust Normalized Percent Inhibition (NPI), % of negative controls, Z-score.
  • Median-based normalizations are called "Robust" because they are not heavily influenced by extreme outliers.​

Notes:

• A second readout can be added so that two inputs are used for a simple calculation, allowing the normalization feature to be computed.
• The [RUN] button remains disabled until all Analytics parameters are configured.