Spectrus: Using peak purity assessment to detect co elution

Description
Even when chromatographic peaks appear well‑resolved, they may contain contributions from more than one component. Peak purity assessment based on spectral changes across the peak can help detect co‑elution.

Solution

1. Import LC/UV or PDA (DAD) data into Spectrus.
2. From the Analysis menu choose Peak Matching>Options and adjust settings.
3. From the Analysis menu choose Peak Matching>Run.

4. View Table of Peaks and observe results in Peak Purity column.

   

   The software compares UV spectra across the peak.

   Peak purity refers to how similar the spectra are when taken from both sides of a broad peak. If the peak purity value is high, this indicates that the peak contains only one component, and the result shown in the Table will be labeled as Pure. If the peak purity value falls below a certain threshold, the software displays a numerical value instead and may split the peak into two or three separate peaks. For peaks with numerical values, it’s important to review them carefully and adjust the peak detection results.