Irina Oshchepkova
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Articles
Recent activity by Irina Oshchepkova-
AutoChrom: Factors affecting recommended New Gradient Conditions
DescriptionWhen an AutoChrom project enters the Optimization wave, the initial processed dataset—based on the original gradient conditions—is carried over from the Screening wave or generated and p...
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AutoChrom: Algorithm fails to recommend two New Gradient Conditions for Optimization wave
DescriptionAfter the user completes Screening and selects the best experiment, the selected condition and its processed data are passed to the Optimization wave (provided the strategy includes an o...
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LC Simulator: Simulated chromatograms differ greatly from experimental data
DescriptionUsers run LC Simulator (or an AutoChrom, LC simulation module) and find that simulated retention times and peak shapes are far from their experimental chromatograms. Investigation shows ...
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Spectrus: No peaks displayed on the chromatogram in Spectrus due to selection of non signal channel during import
DescriptionAfter importing LC data into Spectrus, the chromatogram may appear without peaks even though clear signals are visible in the vendor software. This typically occurs when a non‑analytical...
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Spectrus: Using peak purity assessment to detect co elution
DescriptionEven when chromatographic peaks appear well‑resolved, they may contain contributions from more than one component. Peak purity assessment based on spectral changes across the peak can he...
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LC Simulator: using “Number of Steps” and “Run Count” values for enhanced Model robustness
DescriptionLC Simulator can suggest a minimum number of experiments needed for modeling (for example, for gradient optimization). However, this minimum guarantees only that the model can be built m...
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Spectrus Processor: How to connect to Waters UNIFI using “Connect To”
DescriptionThe Connect To function for Waters UNIFI requires authentication and server details. The connection is made through the UNIFI web API, not directly to the database, which addresses conce...
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AutoChrom: Using software in a validated environment (general guidance)
DescriptionIn environments where instruments and chromatography data systems are fully validated, AutoChrom is often used on separate, non‑instrument PCs. IT or validation groups may perform their ...
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LC Simulator: Modes Manager: “Approximation” vs. “Spline” and required run count
DescriptionIn Modes Manager (for example, Gradient Optimization), some models use polynomial approximation (e.g., quadratic: ln k' = a + bx + cx²), while others use spline models. The “Requires … r...
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LC Simulator: Meaning of “Flow Rate” fields and how t₀ is calculated
DescriptionIn LC Simulator there are two separate flow rate fields:“At a Flow Rate of” (in Column Parameters): flow rate used when the Observed t₀ and Observed Pressure values were measured.“Consta...