Spectrus: Handling exchangeable protons (OH, NH) in NMR assignment and integration

Description
Exchangeable protons (OH, NH) may broaden, shift, or disappear depending on solvent, temperature, and conditions (e.g., D2O exchange). This complicates integration and assignment.

Solution

1. Identify likely exchangeable protons based on the structure (OH, NH, SH).
2. Acquire spectra under conditions appropriate to observe or suppress these protons:
   • For observation: non‑exchangeable solvents, minimal water, moderate temperature.
   • For suppression: D2O exchange experiments if needed.
3. In Spectrus:
   • Label exchangeable signals as OH, NH, etc., and note possible variability.
4. For integration:
   • Treat integrals of exchangeable peaks with caution; they may not reliably reflect proton count.
   • Prefer using non‑exchangeable peaks for stoichiometric calculations.
5. Document in reports that certain signals are exchangeable and can vary with conditions.