Spectrus: Handling polarity switching data (positive and negative mode) in xC/MS

Description
Some xC/UV/MS methods use polarity switching, producing both positive and negative ion data within one run. Spectrus can separate and analyze these data streams.

Solution

1. Import the xC/UV/MS dataset into Spectrus.
2. Check the channel list for:
   • Positive‑mode TIC and associated spectra.
   • Negative‑mode TIC and associated spectra.
3. View and process each polarity separately:
   • Select the positive TIC and related spectra to analyze cationic species.
   • Select the negative TIC and spectra to analyze anionic species.
4. For each component of interest:
   • Determine which polarity gives the best response.
   • Create XICs per polarity if needed.
5. Clearly indicate polarity in all annotations and reports:
   • For each m/z, note whether it was observed in positive or negative mode.
6. Use both polarities together to gain a complete view of the sample’s ionizable species.