Description
After importing and processing an xC/UV/MS dataset, users see chromatographic peaks clearly in TIC or UV traces, but the Component Table (or equivalent) remains empty. This often occurs when automatic component detection was not run, thresholds are too strict, or only certain channels are processed.
Solution
- Confirm which signal is used for component detection:
- Check processing settings used for peak picking and integration (for example, under Signal Analysis: Auto or IX 2.0 in the Analysis tools section):
- Are components detected from TIC, specific XICs, UV channels, or a combination?
- Ensure the appropriate channel (e.g., TIC or main UV wavelength) is enabled for detection.
- Check processing settings used for peak picking and integration (for example, under Signal Analysis: Auto or IX 2.0 in the Analysis tools section):
- Run component detection explicitly
- In Spectrus, locate the Detect Components, Find Peaks, or similar function (for example, under Signal Analysis: Auto or IX 2.0 in the Analysis tools section):
- Run the detection step with default or method‑specific parameters.
- Adjust thresholds and minimum peak settings
- If the table is still empty or missing obvious components, review:
- Minimum peak height or area thresholds.
- Minimum peak width.
- S/N criteria, if available.
- Lower thresholds cautiously to allow detection of smaller peaks, then rerun detection.
- If the table is still empty or missing obvious components, review:
- Check that you are viewing the correct level (per injection or per batch)
- Some workflows distinguish between injection‑level and batch‑level component tables.
- Make sure the correct sample/injection is selected in the UI when inspecting the component list.
- Save processing method for reuse
- Once parameters produce a sensible component table:
- Save these settings as a processing method.
- Apply the same method to future datasets for consistent component detection.
- Once parameters produce a sensible component table:
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